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ll lysis answer containing: NaCl, 150; Tris-HCl, 10; EDTA, 1; SDS, 1%, as well as a protease inhibitor cocktail mixture. The purity of mitochondrial preparations was checked as adhere to. Protein extracts from each isolated mitochondria and complete tissues were divided into 4 aliquots that have been resolved by SDS-PAGE on 8% polyacrylamide gels and then analyzed by western blot with goat anti-ca
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ll lysis answer containing: NaCl, 150; Tris-HCl, ten; EDTA, 1; SDS, 1%, along with a protease inhibitor cocktail mixture. The purity of mitochondrial preparations was checked as adhere to. Protein extracts from both isolated mitochondria and whole tissues were divided into 4 aliquots that were resolved by SDS-PAGE on 8% polyacrylamide gels after which analyzed by western blot with goat anti-calnex
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